Pgk promoter sequence

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For this promoter, there is a cluster of 2 transcription start sites,in the interval 3072928 to 3072929, where the highest frequency is at position 3070951. The most upstream position of this cluster is assigned as TSS position in the promoter. Lentiviral vector with internal CMV promoter, has IRES-ZsGreen (IRES-fluorescent tag); amp resistance; restriction enzyme cloning. Synonyms: pHAGE-CMV-IRES-ZsGreen, pHAGE-CMV-MCS-IRES-ZsGreen-W Sep 28, 2007 · under the control of the murine phosphoglycerate kinase (PGK) promoter (P PGK) for the selection of stable transductants. The vector also contains a pUC origin of replication and an E. coli ampicillin resistance gene (Ampr) for propagation and selection in bacteria. pLVX-Puro 8102 bp Ampr 5' LTR 3' LTR RRE PBS Ψ cPPT WPRE P PGK Puror MCS P CMV ... Mar 21, 2002 · Transcription does not take place from processed pseudogenes, since they lack the crucial 5’-promoter sequence as well as introns, which may also contain transcription initiation sites. Processed pseudogenes, however, contain 3’- polyadenylation tract and flanking direct repeats , sequences presumably involved in the process of insertion ... The analysis of transformed strains in which single copies of lacZ fusions have been targeted to the qutE locus has identified three constitutive positively acting sequence elements in the pgk gene. Firstly, sequence located between -161 and -120bp (possibly spanning -120) relative to the transcript start site is essential for expression, thus demonstrating that the putative core promoter, including potential TATA and CCAAT boxes, and a pyrimidine rich region, is not alone sufficient to ...

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pLEX-MCS PGK promoter Sequences (1) Addgene Sequences: Full (1) Full Sequences from Addgene (1) Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence). ...
After blast, this promoter sequence is equal to the "3109bp……3628bp" in GenBank: KT351864.1. But I found another PGK promoter in a patent, which sequence is equal to the "127bp……518bp" in Gene Bank...
The prokaryotic promoter gb2 is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the normally used Tn5 promoter. The promoter of the mouse Phosphoglucokinase gene (PGK) is used as eukaryotic promoter. A synthetic polyadenylation signal terminates the kanamycin/neomycin expression.
PGK promoter - neo may influence a nearby gene remove the selection cassette to avoid this potential problem Unexpected phenotype lethal phenotype may result prior to the developmental stage of interest Targeted allele PGK-Neo
Regulated mammalian Protein expression and incorporate regulatory promoter sequences upstream of the Protein-coding region (1) Simplified cloning of DNA generated by PCR using any type of DNA polymerase (1) Synthesis of RNA in-vitro and allows selection of recombinants by blue/white screening (1) Target gene cloning and expression (1)
This sequence cooperates with DMPK promoter elements, one of which binds Sp1, to up-regulate transcription in myoblasts. These data, in conjunction with other studies showing MyoD responsiveness of the DMPK transcript ( 15 , 16 , 58 ), implicate this myogenic factor in regulating transcription of this gene in muscle.
Sequence processing i: The displayed sequence is further processed into a mature form. This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage ...
Lentiviral vector with internal CMV promoter, has IRES-ZsGreen (IRES-fluorescent tag); amp resistance; restriction enzyme cloning. Synonyms: pHAGE-CMV-IRES-ZsGreen, pHAGE-CMV-MCS-IRES-ZsGreen-W
En2 En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence) 13 En2 Gene symbol Gene name Chr. Allele symbol Allele name Promoter Frt yeast FRT (flippase recombination target) site 13 Frt
The vectors also contain a promoter that is active in ES cells [such as that of the mouse phosphoglyceratekinase (Pgk) gene] followed by a first exon (such as that of the Bruton’s Tyrosine Kinase (Btk) gene) upstream of a splice donor (SD) signal.
Operon: Localization ofthe Promoter and Identification of a Conserved Sequencein the Regulatory Region WENDY0. BARNELL,1JIANLIU,' TINAL. HESMAN,1MICHAELC.
pHAGE-PGK-MCS-IRES-ZsGreen-W Description: Lentiviral vector that expresses insert from internal PGK promoter, after MCS for insert is IRES-ZsGreen fluorescent marker, with WPRE; ampicillin resistance; restriction enzyme cloning.
The prokaryotic promoter gb2 is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the normally used Tn5 promoter. The promoter of the mouse Phosphoglucokinase gene (PGK) is used as eukaryotic promoter. A synthetic polyadenylation signal terminates the kanamycin/neomycin expression.
Vector Sequence: Sequence: ... promoter H1 H1 promoter ... PGK PGK promoter 1768 1370 selectable marker ...
Vector with a minimal promoter and an NFAT response element for studying cell signaling using destabilized luciferase.
Jan 06, 2009 · Target genomic sequences were amplified with specific primer sets by qPCR. Left, the genomic region adjacent to the SARE sequence (within 200 bp) was detected in the immunoprecipitates obtained using an anti-CREB antibody, while a negative control sequence 10 kb downstream of SARE was not. The Somatostatin promoter was used as a positive control.
sequence yeast promoter dna coding sequence Prior art date 1989-10-18 Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Expired - Lifetime Application number JP28055790A Other languages Japanese (ja) Other ...
Expression of reporter gene in neurons transduced with lentiviral vector under phosphoglycerate kinase (PGK) promoter. Mixed murine neocortical cultures containing both neurons and astrocytes were transduced with lentiviral vector PGK-GFP at an MOI of 5. Cultures were immunostained and imaged 7 days after transduction.
Minimal Promoter-Driven NanoLuc® Reporter Vectors. Two vectors that offer a minimal promoter for cloning response elements of interest to express NanoLuc® luciferase. N1031, N1041. Promoter-Driven Control NanoLuc® Luciferase Vectors. Three NanoLuc® reporter vectors with CMV, PGK or TK promoter.

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Liver toxicity is the major concern for use of recombinant human tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) proteins in treatment of cancers. Here we report that normal human primary hepatocytes (NHPHs) are susceptible to the transduction of the wild-type, full-length coding sequence of the human TRAIL gene. To minimize potential toxicity of the TRAIL gene, a bicistronic ...
Here we used postnatal day 1 systemic injection of self-complementary adeno-associated virus (scAAV9) vectors carrying a codon-optimized SMN1 sequence and a chimeric intron placed downstream of the strong phosphoglycerate kinase (PGK) promoter (SMNopti) to overexpress the human SMN protein in a mouse model of severe SMA.
Jun 09, 2018 · Its 5' to 3' sequence is the same as in the database (ACCESSION AE000141). (b) Presented so that the promoter sequence numbers increase from left to right. Its 5' to 3' sequence is comparable to the consensus sequence. 16. lac promoter- lactose- (or IPTG- isopropylthiogalactoside) inducible Problems with lac promoter- 1. Weak 2.
quat, stimulates the PGK promoter in yeast. Using a reporter gene construct with the yeast PGK promoter sequence, we show that sublethal, as opposed to lethal, doses ofPQ result in increased stimulation of the PGK promoter at midlogarithmic phase of growth. Addition of the antioxidant N-acetylcysteine (NAC) abolishes this stimulatory effect.
Promoters are about 100-1000 base pairs long and are adjacent and typically upstream (5') of the sense or coding strand of the transcribed gene. The coding strand is the DNA strand that encodes codons and whose sequence corresponds to the mRNA transcript produced.
Information2; Donor DNA: synthetic splice acceptor sequence, P1 phage loxP sites, STOP (mouse phosphoglycerate kinase promoter (PGK promoter), E.coli Neo, polyA), Simian virus 40 Large T antigen nuclear localization signal (NLS), E.coli lacZ, SV40 polyA signal, mouse ROSA26 genomic DNA
Plasmid PGK-H2BmCherry from Dr. Mark Mercola's lab contains the insert Phosphoglycerate kinase promoter and is published in PLoS ONE. 2009 . 4(4):e5046. This plasmid is available through Addgene.
Lentiviral vector with internal CMV promoter, has IRES-ZsGreen (IRES-fluorescent tag); amp resistance; restriction enzyme cloning. Synonyms: pHAGE-CMV-IRES-ZsGreen, pHAGE-CMV-MCS-IRES-ZsGreen-W
Our analysis revealed that PGK has an upstream activation sequence (UASPGK) located between 402 and 479 nucleotides upstream from the initiating ATG sequence which is required for full transcriptional activity. Deletion of this sequence caused a marked reduction in the levels of PGK transcription.
The respective transcriptional initiation sites in front of gap and pgk were located, and, from the analysis of DNA sequences upstream of these and of previously determined transcriptional start sites, common structures which may be important for promoter function in C. glutamicum are discussed.
For this promoter, there is a cluster of 2 transcription start sites,in the interval 3072933 to 3072934, where the highest frequency is at position 3070956. The most upstream position of this cluster is assigned as TSS position in the promoter.
Packaging Plasmids: Plasmid Name: Description: Map: Sequence: Data sheet: pCAG-HIVgp: CAG promoter, HIV-1 gag and pol: pCAG-HIVgp IN-D64V: CAG promoter, HIV-1 gag and pol, integrase mutant
significantly higher percentage of sequences align to the 500-bp segment upstream of the transcription start sites of known genes than would be expected from random genomic sequences. We also observed enrichment for putative promoter regions of genes predicted in at least two annotation databases and for clones overlapping with CpG islands.
The results revealed that the 1.4 kb DNA region is sufficient for determining the organ‐specific, developmental stage‐specific and spermatogenic stage‐specific transcription of the mouse Pgk‐2 gene. When the region between – 684 and + 61 was used to generate transgenic mice, β‐galactosidase mRNA was detectable not only in the testis, but also in other organs such as brain and lung.
Jan 02, 2015 · Li W, Li DM, Chen K, et al: Development of a gene therapy strategy to target hepatocellular carcinoma based inhibition of protein phosphatase 2A using the α-fetoprotein promoter enhancer and pgk promoter: an in vitro and in vivo study. BMC Cancer. 12:5472012. View Article: Google Scholar. 9



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